Saliva Hormone Tests

by ZRT Laboratory

Saliva hormone tests reflect the tissue concentration of your hormones.  Blood and serum tests contain the total hormone, but your tissue are only bathed with the active or effective fraction as found in saliva.  Active fraction measurements are superior to blood and urine measurements both in diagnosis and treatment.

Healthway Compounding Pharmacy uses ZRT Laboratory for saliva diagnostic purposes.


It may be useful to review hormone imbalance symptoms.  Please review hormone symptoms list, before deciding which hormone test to order.

For a comprehensive overview of your hormone status, ZRT Laboratory recommends estradiol, progesterone, testosterone, DHEAs, and cortisol (AM/PM).

If you suffer from excessive fatigue, depression, and anxiety ZRT Laboratory recommends you test cortisol both in the morning and evening.

If you are supplementing with a hormone, ZRT Laboratory advices you take the saliva test for that hormone.  For example, test estriol if you are using a vaginal estriol cream – also test estradiol.

Each test includes a short personal consultation either over the phone or in person.


Hormones Available for Testing (Saliva Collection):

AM Cortisol (C)

Cortisol is a hormone produced by the adrenal glands that regulates the stress response and many biochemical functions including glucose  metabolism and immune function.  Cortisol has a catabolic (breaking down) action on the body when levels are too high or out of balance. This test is recommended with situational stress and fatigue; indicator of adrenal imbalance.

AM/PM Cortisol

Recommended with excess stress, morning and evening fatigue, difficulty sleeping, etc.; indicator of adrenal dysfunction.

Adrenal Function Test (AFT)

Adrenal imbalance or low adrenal reserve is a result of excessive stress, overworked adrenal glands fail to produce balanced levels of the hormones cortisol and DHEA.  Adrenal exhaustion occurs when the adrenals no longer produce enough hormone to meet your body’s demand, a result of prolongs stress. The adrenal function test is the best comprehensive assessment of adrenal gland function.  It is recommended with excessive stress, chronic fatigue, sleep disorders and allergies.  It is an indicator of immune function.  Tests include DHEA’s and four cortisols: morning, noon, evening, and night.


Dehydroepiandrosterone (DHEA) is a hormone made mostly by the adrenal glands.  It converts into androgens, like testosterone and estrogens.  Its actions influence energy, stamina, mental outlook and immune function.  DHEA testing is recommended with androgen deficiency or excess symptoms.  It is an indicator of stress level, mental performance an insulin resistance.

Estradiol (E2)

Estradiol is the most predominant of the estrogens.  Testing is recommended with symptoms of estrogen deficiency or excess.  Estradiol is best tested in combination with progesterone since an imbalance of these two hormones is associated with menopause symptoms and disorders of the reproduction system.  It is recommended that Estradiol levels be measured when using a compounded prescription of estrogens containing Estradiol.

Estriol (E3)

Estriol levels should be measured when using a compounded prescription of estrogens containing Estriol.

Hormone Profile I ( 5 Tests)


Good basic assessment of overall steroid hormone balance or imbalance and association with specific symptoms.  It is an indicator of hypthryrodism, bone loss and insulin resistance.  Kit includes: Estradiol (E2), Progesterone (Pg), Testosterone (T), DHEA’s, and morning Cortisol (C).

Hormone Profile II + PM Cortisol ( 6 Tests)

Hormone Profile II is the most comprehensive evaluation of overall hormone balance or imbalance.  It’s an indicator of thyroid and adrenal function; breast cancer profile.  The kit includes all the tests in Hormone Profile I plus PM Cortisol (C4).

Hormone Profile III + AFT ( 8 Tests)

Hormone Profile III provides the most complete and in-depth evaluation of hormone balance, imbalance and interrelationship of specific hormone levels as related to symptoms of menopause, andropause and adrenal function.  Kit includes eight test:  Estradiol (E2), Progesterone (Pg), Testosterone (T), DHEA’s and ‘Diurnal Cortisol’ (all four cortisols: morning (C1), noon (C2), evening (C3), and night (C4).

Progesterone (Pg)

Progesterone testing is recommended with symptoms of deficiency or excess.  It is best tested in conjunction with estradiol as an imbalance of these two hormones is associated with menopause symptoms and disorders of the system.

Testosterone (T)

Testosterone is an anabolic or “building” hormone that possess a unique chemical structure designed to maintain lean body mass and bone density, while facilitation skin elasticity, sex drive and cardiovascular health in both men and women.  The Testosterone test is recommended with deficiency or excess symptoms.  It is an indicator of low sex drive, hair loss/excess, muscle and bone status.

Before deciding which hormones or hormone profiles to test, please review hormone symptoms list. Two or more persistent symptoms in any given category is a strong indication of hormonal imbalance and the need to test levels of that specific hormone or profile .


  • Hormone Users: You do not have to go off hormone therapy to take the test; please refer to “Men or Women Taking Hormones” in your test kit instructions for details of when to collect while supplementing. We do advise testing those hormones you are currently being supplemented with for purposes of monitoring safety and efficacy.


  • For a comprehensive overview we recommend:  
    • Hormone Profile II – This tests estradiol,( the most potent of the estrogens) progesterone, testosterone, DHEA-S, and cortisol (AM/PM). 
  • For symptoms of Adrenal Imbalance such as excessive fatigue, depression, allergies and/or anxiety we recommend: 
    • AM/PM Cortisol, in Saliva. This test measures cortisol levels twice in the same day (morning and evening).
    • Adrenal Function Test is recommended for moderate to severe symptoms. This profile evaluates complete adrenal function with 4-same day (morning, noon , evening, bedtime) cortisol collections. To ensure accuracy, cortisol collections should take place two-hours apart from food or drink other than water. 


More Information
Saliva hormone testing is available without a prescription through Healthway Compounding Pharmacy. After receiving your saliva test kit, follow the directions specified for your particular test. After collection you will send the test into ZRT Laboratory for testing. The results will be sent to Healthway Compounding Pharmacy and interpreted by one of our highly-trained pharmacists. Your results will be explained during a private consultation with our pharmacist, either  by phone or in person. If you wish, a copy of your test results can be sent to your physician. For more information on Saliva Hormone Testing, contact Healthway Compounding Pharmacy at 989-791-1691.



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